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hTGIT

1. 基本信息

品系名稱:B6-Tigitem1(hTigit)Vst/Vst

常用名C57BL/6-Tigit人源化小鼠; Tigit人源化小鼠;Hu-Tigit

背景C57BL/6NCrl

毛色黑色


品系建立

采用CRISPR-Cas9辅助的方法,将小鼠Tigit基因(T Cell Immunoreceptor With Ig And ITIM Domains)替换为人源Tigit。这样在Tigit人源化小鼠体内,使用人源Tigit抗体抑制剂时就可以识别,进行相关的药效或药物筛选实验。


2. 应用领域

-人源化和癌症治療

-免疫治療

-藥物篩選


3. 参考文献

(1)The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.Yu X, Harden K, Gonzalez LC, Francesco M, Chiang E, Irving B, Tom I, Ivelja S, Refino CJ, Clark H, Eaton D, Grogan JL.Nat Immunol. 2009 Jan;10(1):48-57. doi: 10.1038/ni.1674. Epub 2008 Nov 16.

(2)Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering.Stengel KF, Harden-Bowles K, Yu X, Rouge L, Yin J, Comps-Agrar L, Wiesmann C, Bazan JF, Eaton DL, Grogan JL.Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5399-404. doi: 10.1073/pnas.1120606109. Epub 2012 Mar 15.